Figure 4. Effects of mapracorat and
dexamethasone on CXCR4 receptor and annexin I surface expression
in human eosinophils. Eosinophils were routinely cultured in 10%
fetal bovine serum containing prosurvival granulocyte-macrophage
colony stimulating factor and interleukin-5 (controls);
alternatively, eosinophils were maintained for 48 h in 0.1%
fetal bovine serum lacking granulocyte-macrophage colony
stimulating factor and interleukin-5 and were treated with
mapracorat or dexamentasone (0.01–10 μM) or their vehicle.
C-X-C-chemokine receptor 4 (CXCR4) or annexin 1 expression was
evaluated by flow cytometry analysis as described under Methods.
CXCR4 receptor expression (A) and annexin I expression (B)
are presented as percentages of positive cells and calculated as
described under Methods. Data are presented as mean±standard
error of the mean from six experiments performed in triplicate
using different eosinophil cell cultures. **Versus vehicle; p
(value) p<0.01. §Versus dexamethasone 1 μM;
p<0.05. §§Versus dexamethasone 5 and 10 μM;
p<0.01. Abbreviations: Ctrl represents controls.