Figure 4. Effects of mapracorat and dexamethasone on CXCR4 receptor and annexin I surface expression in human eosinophils. Eosinophils were routinely cultured in 10% fetal bovine serum containing prosurvival granulocyte-macrophage colony stimulating factor and interleukin-5 (controls); alternatively, eosinophils were maintained for 48 h in 0.1% fetal bovine serum lacking granulocyte-macrophage colony stimulating factor and interleukin-5 and were treated with mapracorat or dexamentasone (0.01–10 μM) or their vehicle. C-X-C-chemokine receptor 4 (CXCR4) or annexin 1 expression was evaluated by flow cytometry analysis as described under Methods. CXCR4 receptor expression (A) and annexin I expression (B) are presented as percentages of positive cells and calculated as described under Methods. Data are presented as mean±standard error of the mean from six experiments performed in triplicate using different eosinophil cell cultures. **Versus vehicle; p (value) p<0.01. ^§Versus dexamethasone 1 μM; p<0.05. ^§§Versus dexamethasone 5 and 10 μM; p<0.01. Abbreviations: Ctrl represents controls.