Figure 5. Supplementation with lutein, zeaxanthin or α-tocopherol suppressed H₂O₂-induced DNA damage in human lens epithelial cells (HLEC). Subconfluent HLEC were incubated with or without 5 µM of lutein, zeaxanthin or α-tocopherol for 48 h and then exposed to 100 µM H₂O₂ for 1 h. A: Fluorescence micrograph of nuclei of normal HLEC. B: Fluorescence micrograph of nuclei of oxidative insulted HLEC. C: Quantification of DNA damage. Each value represents the mean±SD (n=6). *Indicates a p<0.01 when comparing with cells not treated with H₂O₂ and **indicates a p<0.05 comparing with unsupplemented cells that were exposed to the same level of H₂O₂.