Figure 2. Dose-dependent effects of lutein supplementation on protein and lipid oxidations. Subconfluent human lens epithelial cells were incubated with 0, 1, or 5 µM lutein for 48 h to allow the cells to accumulate lutein. The cells were then treated with or without 100 µM H₂O₂ for 1 h. Levels of protein carbonyl and MDA in the cells were determined as described in the “Methods.” A: The effects of lutein supplementation on protein carbonyl in HLEC that were not exposed to H₂O₂. B: The effects of lutein supplementation on protein carbonyl in HLEC that were exposed to 100 µM H₂O₂ for 1 h. C: Densitometry quantification of western-blotting results in B (n=3). D: The effects of lutein supplementation on MDA levels (n=6), *Indicates a p<0.05 when comparing H₂O₂-exposed groups to the control group that were not treated with H₂O₂ and **indicates a p<0.05 when comparing lutein supplemented groups to the unsupplemented group upon exposure to 100 µM H₂O₂ for 1h.