Figure 1. Localization of intravitreally injected fibronectin (FN)-SiRNA in vascular cells of a rat retina. FN-siRNA injected intravitreally in rat eyes localized on the inner limiting membrane and in retinal microvessels. The arrow points to a microvessel showing an endothelial cell nucleus (4',6-diamidino-2-phenylindole [DAPI]-positive) in panel A, and panel B shows Cyanine-3 labeled FN-siRNA localized in the same endothelial cell of the retinal microvessel. Panel C shows a superimposed image of A and B. Panel D shows a close-up view of the retinal microvessel shown in panel C. Bar: 50 μm. Inner limiting membrane (ILM); Retinal capillary (RC).