Figure 5. The LT50 of the four anti-inflammatory eye drops. HCECs were treated directly with 100-μl undiluted eye drops and stopped by 10-mL DMEM/F-12 media (n=4). The cells were labeled with 7-AAD staining, and the cell viability was measured using flow cytometry. The LT50 was counted according to the cell viability curve. The LT50 was 23.80 min for Bromfenac Sodium Hydrate Eye Drops, 10.38 min for Pranoprofen Eye Drops, 9.23 min for Diclofenac Sodium Eye Drops, and 3.28 min for Tobramycin & Dex Eye Drops.