Figure 5. Changes in MAPK activation patterns and IL-6/8 release in DUSP1 subline. A: Western blot analysis of total DUSP1 protein expression in resting scrambled HCEC and DUSP1 subline. B: Comparison of time dependent changes in MAPK phosphorylation in scrambled shRNA and DUSP1subline. Cells were exposed to CAP (20 µM) for indicated times. Changes are compared in p-ERK1/2, p-JNK1/2, and p38 MAPK in two different sublines. Protein loading equivalence validated based on invariant ERK1/2 expression levels. C: DUSP1 gene knockdown enhances CAP-induced IL-6 and IL-8 release. ELISA was performed on scrambled shRNA and DUSP1 subline after 24 h exposure to CAP (20 µM). Three independent experiments each performed in triplicate.