Figure 2. Dependence of CAP-induced increases in IL-6 and IL-8 release on JNK1 activation. A: Validation of JNK1 knockdown. Western blot analysis compares total JNK1 protein expression in parental wildtype (WT), resting scrambled shRNA and JNK1 HCEC subline. Expression levels in scrambled and WT HCEC were the same. JNK1/2 expression level invariance validates protein loading equivalence. B: Loss of JNK1 expression obviates IL-6/8 responses to TRPV1 activation. ELISA was performed on scrambled shRNA and JNK1 subline after 24 h in the presence or absence of CAP (20 µM). Results are from three independent experiments each performed in triplicate.