Table 2 of
Saee-Rad, Mol Vis 2011; 17:3128-3136. Table 2 of
Saee-Rad, Mol Vis 2011; 17:3128-3136.
Table 2. List of VSX1 and SOD1 primers and predicted PCR product sizes (bp) used in this study.
Primer |
Sequence (5'→3') |
PCR product size (bp) |
Primer location |
|---|---|---|---|
| VSX1 primer sequences | |||
| V1F | 5′-GCAGCCCAATCCTATAAAGC-3′ | 687 | 1–20 |
| V1R | 5′-GATTACCGGACGTGGAGA-3′ | 469–486 | |
| V2F | 5′-AAGTCCTCTTCTTCTTTCTGTGCCATC-3′ | 800 | 2610–2637 |
| V2R | 5′-AAGGGACTGCTGATTGGCTCACTG-3′ | 3386- 3409 | |
| V3F | 5′-ATCATGCTCGGGAGAGAAGA-3′ | 487 | 4178–4197 |
| V3R | 5′-AAAATGAGGCAACCATCCAG-3′ | 4639–4660 | |
| V4F | 5′-CCAATGCCAATCACTGTGTC3′ | 306 | 5366–5385 |
| V4R | 5′-CCCAGAGTCCTGCCAACTTA-3′ | 5652–5672 | |
| V5F | 5′-AGGAAGTGAAGATAAGTTGGCAG-3′ | 470 | 5640–5662 |
| V5R | 5′-TAAAGTGCCATTAAGGAACCG-3′ | 6110–6090 | |
| V6F | 5′-AACGGTTCCTTAATGGCACTT-3′ | 301 | 6088–6109 |
| V6R | 5′-TTGAAATATCCAAGGCCAAGTT-3′ | 6367–6388 | |
| V7F | 5′-ATCATAGTGAAGACTCCATACAGACA-3′ | 424 | 6312–6337 |
| V7R | 5′-AGCCCTCACAATGAGCAGTT-3′ | 6964–6984 | |
| V8F | 5′-GAGGCAGCATCTCAGGACTT-3′ | 534 | 9834–9854 |
| V8R | 5′-AGGTGTGAGGTACAGGTCCAA-3′ | 9321–9340 | |
| V9F | 5′-GCTCAGGTAGCATTGTTCTGC-3′ | 610 | 10272–10292 |
| V9R | 5′-TGATGGAAGGAGAGGAGAAGG-3′ | 10861–10881 | |
| ARMS primers | |||
| VSM | 5′-AGTCTGGCAGCGAGATGTAGC-3′ | 236 | 4392–4411 |
| VSWT | 5′-ACTGCATCCCGCTGCCAGACT-3′ | 43911–4411 | |
| VSF | 5′-GGATCATGCTCGGGAGAGAAGA-3′ | 4176–4197 | |
| SOD1 primer sequences | |||
| S1F | 5′-CTCCACATTTCGGGGTTCT-3′ | 450 | 4850–4868 |
| S1R | 5′-ACCCGCTCCTAGCAAAGGT-3′ | 5281–5292 | |
| S2F | 5′-CCATCTCCCTTTTGAGGACA-3′ | 426 | 8965–8985 |
| S2R | 5′-CGACAGAGCAAGACCCTTTC-3′ | 9371–9390 | |
| S3F | 5′-TGATGCAGGTCAGCACTTTC-3′ | 344 | 11717–11736 |
| S3R | 5′-AAAAGCATTCCAGCATTTGG-3′ | 12041–12060 | |
| S4F | 5′-CCATCTTTCTTCCCAGAGCA-3′ | 386 | 12810–12840 |
| S4R | 5′-GAAACCGCGACTAACAATCAA-3 | 12454–12473 | |
| S5F | 5′-TTTGGGTATTGTTGGGAGGA-3′ | 675 | 13780–13799 |
| S5R | 5′-TCTGTTCCACTGAAGCTGTTT-3′ | 14334–14355 | |