Table 1 of Sodi, Mol Vis 2011; 17:3078-3087.

Table 1. Primers used for the PCR amplification of the BEST1 gene.



Exon

Sense primer (5′-3′)

Antisense primer (5′-3′)		 Annealing
temperature
(°C)
1 ACCCAACACCCTCCAAGAA AGTCCACCTGGGGCACCTT 60
2 CCCTACAAACCCCCAATCG CCAGCCACATCCTTCCCAG 60
3 AGTCTCAGCCATCTCCTCGC TGGCCTGTCTGGAGCCTG 60
4 AGAAAGCTGGAGGAGCCGA TTCACCCATCTTCCATTCCT 58
5 GCCATCCCTTCTGCAGGTT GCGGCAGCCCTGTCTGTAC 62
6 GGGGCAGGTGGTGTTCAGA GGCAGCCTCACCAGCCTAG 60
7 TGATTTCAGGGTTCCCACCTAG CATCCTCGTCTCAGGCAGCT 65
8 AGGGTTTACAGAGCCTCACCTG CACTTTGGGGAAGGTCCATG 60
9 CCTCCAAGTCATCAGGCACATA CTAGGCAGACCCCTGCACTAG 65
40817 ACTGGCTCAGCCCTGCATC GTGGGGCACTGTAGTAGCCTG 60
40818 CCTTCAAGTCTGCCCCACTG TGCAGTGCCCCTGGTTC 60
11 GGTACCTTCCATACTTATGCTG AGGTCTTGGGATGAATCAGA 60

For each exon the forward primer, the reverse primer, and the annealing temperature are indicated.

Sodi, Mol Vis 2011; 17:3078-3087. http://www.molvis.org/molvis/v17/a331

©2011 Molecular Vision http://www.molvis.org/molvis/

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