Figure 3. Effects of PD98059 on the expression of CXCL8, CCL2, and IL-6 in ARPE-19 cells simulated with or without different concentration IL-17A. Cells were cultured with or without 50 μΜ PD98059 (PD) for 2 h, followed by incubation with or without 1, 10, and 50 ng/ml IL-17A for 24 h. Secreted cytokines in supernatants were determined by ELISA. Values are expressed as the mean±SD of four independent experiments. *p<0.05, compared with the values in the absence of inhibitors.