Figure 2. Effects of menadione on mitochondrial integrity. Representative confocal micrographs of mitochondria in secondary fiber cells for 0 µM (A-C), 50 µM (D-F), 200 µM (G-I) and 600 µM (J-L) menadione-treated lenses at 4 h, 24 h, and 48 h. Scale bar represents 20 µm. Note the increase in the number of smaller mitochondria as menadione concentration and time increase.