Figure 3. Comparison of the electrophoretic mobility of HSF4b and HSF4b^lop11 proteins under non-denaturing and denaturing conditions. Nuclear extracts from cells transfected with Hsf4b and Hsf4b^lop11 were separated by A, non-denaturing PAGE or B, SDS/PAGE and immunoblotted with anti-His antibody. The homotrimers could only be detected with HSF4b (arrow) as show in A.The same nuclear extracts as shown in A when run under denaturing conditions exhibited presence of the monomeric HSF4b and HSF4b^lop11 subunits as shown in B. The molecular mass is indicated to the left of each blot.