Differential expression of genes identified in three global profiling studies (Corbo, Mustafi and this report) was validated by qRT–PCR. Genes shown under the Corbo and Mustafi subheadings were identified in respective studies, but not in the current study. Corbo-Mustafi subheading indicates genes identified in both Corbo and Mustafi studies but not in the current report. The two genes, Cd8a and Acoxl, identified previously were differentially expressed by qRT–PCR but were filtered out of our sequencing data because of the low FPKM values observed. The gene Abca13 was detected as only expressing the last 7 exons of the annotated sequence. qRT–PCR assays that distinguished between the two isoforms were able to confirm the differential expression of the last 7 Abca13 exons. This gene was filtered out of our sequencing results because of the low FPKM value observed. All genes included in this report subheading were uniquely identified by the current study (and not in Corbo and Mustafi reports). FC, p-val, and FPKM values in this report are derived from the sequencing results reported here. Genes were selected as having the largest fold changes in each category and from current annotation in the latest mm9 Refseq build. All differentially expressed genes identified in this report could be validated by qRT–PCR. FC=fold change, NA=not applicable, FPKM=fragments per kilobase exon model per million reads.