Figure 3. Correlation of RNA-seq and qRT–PCR. The correlations between the RNA-seq fragments per kilobase of exon model per million mapped reads (FPKM) values and the corresponding qRT–PCR crossing threshold (Ct) values are shown. FPKM values represented in log₂ scale, and non-normalized Ct values are an average of three biologic replicates. Data generated from differentially expressed genes (black) is contrasted with data generated from the housekeeping genes (red). The dashed line, associated equation, and goodness of fit value were generated by least-squares regression analysis of the differentially expressed data set. Since a lower Ct value indicates an increased initial amount of target mRNA, an inverse relationship between FPKM and Ct values is expected if a correlation exists.