Table 2 of
Banasiak, Mol Vis 2011; 17:3025-3033. Table 2 of
Banasiak, Mol Vis 2011; 17:3025-3033.
Table 2. Characteristic of primers used for real-time QRT-PCR.
Gene |
Sequence of primers |
Length of amplicon (bp) |
Tm (ºC) |
|---|---|---|---|
| GAPDH | Forward: 5’-GAAGGTGAAGGTCGGAGTC-3’ | 226 | 80 |
| Reverse: 5’-GAAGATGGTGATGGGATTC-3’ | |||
| TGFβ1 | Forward: 5’-TGAACCGGCCTTTCCTGCTTCTCATG-3’ | 151 | 85 |
| Reverse: 5’-GCGGAAGTCAATGTACAGCTGCCGC-3’ | |||
| TGFβ2 | Forward: 5’-TACTACGCCAAGGAGGTTTACAAA-3’ | 201 | 80 |
| Reverse: 5’-TTGTTCAGGCACTCTGGCTTT-3’ | |||
| TGFβ3 | Forward: 5’-CTGGATTGTGGTTCCATGCA-3’ | 121 | 81 |
| Reverse: 5’-TCCCCGAATGCCTCACAT-3’ |