Table 1 of
Li, Mol Vis 2011; 17:2986-2996. Table 1 of
Li, Mol Vis 2011; 17:2986-2996.
Table 1. Primer sequences.
| ID | Name | Sequence |
|---|---|---|
| Primer for RT–PCR/RT-qPCR | ||
| p1f | FRMD7-FL-f | 5′-CAAAGCAGGTAAAAAATCCTAAGG-3′ |
| p1r | FRMD7-FL-r | 5′-ATGTGAGATACCATCAACGCTGT-3′ |
| p2f | FRMD7-S-f | 5′-TAACAAAGCAGGTAAAAATGGAC-3′ |
| p2r | FRMD7-S-r | 5′-AAGATGTGAGATACCATCAACG-3′ |
| p3f | GAPDH-f | 5′- CCCACTCCTCCACCTTTGAC-3′ |
| p3r | GAPDH-r | 5′- CATACCAGGAAATGAGCTTGACAA-3′ |
| Primer for cloning | ||
| pAf | pcDNA3.1-FRMD7-FL-HA-f | 5′-CGCTAGCGCCACCATGCTACATTTAAAAGTGCAGT-3′ |
| pAr | pcDNA3.1-FRMD7-FL-HA-r | 5′-GCTCGAGTTAAGCGTAATCTGGAACATCGTATGGGTAAGCTAAAAAGTAATTACATG-3′ |
| pBf | pcDNA3.1-FRMD7-S-Myc-f | 5′-CGCTAGCGCCACCATGCTACATTTAAAAGTGCAGT-3′ |
| pBr | pcDNA3.1-FRMD7-S-Myc-r | 5′-GCTCGAGTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCAGCTAAAAAGTAATTACATG-3′ |
| pCf | pEGFP-FRMD7-FL-f | 5′-CGCTAGCATGATTCCCAGAAGAT-3′ |
| pCr | pEGFP-FRMD7-FL-r | 5′-GCTCGAGAGCTAAAAAGTAATTACA-3′ |
| pDsRed-FRMD7-S-f | 5′-CGCTAGCATGATTCCCAGAAGAT-3′ | |
| pDr | pDsRed-FRMD7-S-r | 5′-GCTCGAGAGCTAAAAAGTAATTACA-3′ |