Figure 4. Sub-cellular co-localization and co-immunoprecipitation of hFRMD7-FL with hFRMD7-S. Co-transfection of pEGFP-hFRMD7-FL (green) and pDsRed-hFRMD7-S (red) into RA-untreated (A) or differentiating NT2 cells (B). After 48 h, the cells were fixed and DAPI was used as a nuclear counterstain (blue). Magnification 400×. C and D: Reciprocal co-immunoprecipitation experiments show that hFRMD7-FL-HA co-immunoprecipitates with hFRMD7-S-Myc, and vice versa, in NT2 cells. Cells transfected with only hFRMD7-S-Myc or hFRMD7-FL-HA served as the negative control.