Figure 3. Effects of hFRMD7-FL or hFRMD7-S overexpression on neurite outgrowth. NT2 cells stably transfected with hFRMD7-FL-HA (A), hFRMD7-S-Myc (B), or the empty pcDNA3.1(+) vector (C) were treated with ATRA for 5 days. The cells were fixed and immunostained with monoclonal anti-Myc or anti-HA antibodies and a DyLight-488 conjugated secondary antibody. Class III β-tubulin was immunostained using a monoclonal TuJ1 antibody and DyLight-594 secondary antibody. Magnification: 200×.