Figure 1. Cloning of a novel FRMD7 isoform and tissue distribution of the two splice variants. A: Components of the FERM domain and gene structure of hFRMD7-FL. B and C: Sequence comparison between hFRMD7-FL/mFRMD7-FL (B) and hFRMD7-S/mFRMD7-S (C) showing the deletion of 45 bp in the 5′ end of exon 4 in the hFRMD7-S/mFRMD7-S. D: Agarose gel electrophoresis of the RT–PCR products to confirm their size and the identification of a single PCR product. E: Expression levels of hFRMD7-FL and hFRMD7-S transcripts in selected human fetal tissues. The primer sets used in D and E: p1f/p1r, p2f/p2r and p3f/p3r.