Figure 4. Elevated pressure (60 mmHg) induced collapse of cytoskeleton and focal adhesion in both iHTM and GTM₃ after incubation for 24 h. Ctrl: cells cultured in a conventional incubator as the control group. 60 mmHg: cells cultured in the 60 mmHg elevated pressure system. The nucleus was immunolabeled with hoechst (blue fluorescence), filamentious actin (F-actin) was immunolabeled with TRITC (red fluorescence), and Vinculin was immunolabeled with FITC (green fluorescence). Slides were observed under the confocal microscope using identical parameters (scale bar=10 µm).