Figure 5. Glial microenvironment remains intact after RGC transfection using the modified capillary gun. A-C: The astroglial layers of retinal explants transfected with YFP using the modified capillary gun retain their normal, coherent, web-like appearance. D-F: In contrast, those in explants transfected using a conventional entrainment biolistic device show extended regions of astroglial degeneration. Scale bar represents 50 μm.