Table 2 of
Heynen, Mol Vis 2011; 17:2934-2946. Table 2 of
Heynen, Mol Vis 2011; 17:2934-2946.
Table 2. Primers and conditions for real time PCR
| Oligonucleotide primers | ||||
|---|---|---|---|---|
Gene |
Forward 5′-3′ |
Reverse 5′-3′ |
Annealing temperature (°C) |
Product (bp) |
| Cdc42 | GGCGGAGAAGCTGAGGACAAG | AGCGGTCGTAGTCTGTCATAATCCTC | 60 | 275 |
| Gnat1 | GAGGATGCTGAGAAGGATGC | TGAATGTTGAGCGTGGTCAT | 58 | 209 |
| Chx10 | CCAGAAGACAGGATACAGGTG | GGCTCCATAGAGACCATACT | 60 | 111 |
| Opn4 | CCAGCTTCACAACCAGTCCT | CAGCCTGATGTGCAGATGTC | 62 | 111 |
| Actb | CAACGGCTCCGGCATGTGC | CTCTTGCTCTGGGCCTCG | 62 | 153 |
| Rho | CTTCACCTGGATCATGGCGTT | TTCGTTGTTGACCTCAGGCTTG | 62 | 130 |
| Opn1sw | TGTACATGGTCAACAATCGGA | ACACCATCTCCAGAATGCAAG | 58 | 153 |
| Opn1mw | CTCTGCTACCTCCAAGTGTGG | AAGTATAGGGTCCCCAGCAGA | 58 | 154 |
| Lif | AATGCCACCTGTGCCATACG | CAACTTGGTCTTCTCTGTCCCG | 60 | 216 |
| Edn2 | AGACCTCCTCCGAAAGCTG | CTGGCTGTAGCTGGCAAAG | 60 | 64 |
| Fgf2 | TGTGTCTATCAAGGGAGTGTGTGC | ACCAACTGGAGTATTTCCGTGACCG | 62 | 158 |
| Gfap | CCACCAAACTGGCTGATGTCTAC | TTCTCTCCAAATCCACACGAGC | 62 | 240 |
| Stat3 | CAAAACCCTCAAGAGCCAAGG | TCACTCACAATGCTTCTCCGC | 62 | 159 |
| Stat1 | TTGTGTTGAATCCCGAACCT | TCGAACCACTGTGACATCCT | 62 | 95 |
| Socs3 | ATTTCGCTTCGGGACTAGC | AACTTGCTGTGGGTGACCAT | 58 | 126 |