Table 2 of Abu-Amero, Mol Vis 2011; 17:2911-2919.
Exon | Forward (5'-3') | Reverse (5'-3') | Annealing temp. |
---|---|---|---|
1a | CCCAGAGCAGGAGAAAGG | GGAACAGACTGTACACCTTGG | 56 |
1b | GCCCCCTAGACTCAGAGAAG | AATCTTCCAATCCCGATTTT | 58 |
2 | AATGGCAGAGTCAGGATTCA | CTTCAGGACGCAGACTAGGA | 55 |
3 | CTGAGGCCAGGAGAGTGG | CCAGCCCCAACACCTACT | 58 |
4 | AAGCCTGGTGATTCCACATA | CACAAAGCAGGTGCTCAAC | 60 |
5 | GCGTCCAGTAGGTACTCAGC | AGCTAGGCTGCCAAGTGAG | 60 |
6 | GGGGCTGGTTATTATCCACT | GGCTGAGAAGTTGAGGGAAT | 57 |
7 | GGGATCATTCTGGGGTTCTA | CTGTGTGCCTGGTATTGACA | 55 |
8 | ACTCCCTTCTCCCCTTCTTT | ACAGACTGCACCAGCAGAG | 60 |
9 | GCTGAGAGGAGTCTGGTGAG | TGGCTTCCTCTGTCACTCTC | 60 |
10 | GGAGAGGAATCCCACTGAAT | ATCTCTGTTCCAGCAGGATG | 60 |
11 | ATTCCACTACGCCTCTTCCT | GCAGGGAAGGCTACTTCAG | 58 |
12 | ACGTGCTTATCCCAACCTG | TCTTGACCCCATATGGAAGA | 58 |
13 | AAGAGTCCACGCTTTCTGTG | ATGGCTGCTCCATAAACAAG | 60 |
14 | GTAAAGTGCCTGGCAGAATG | GGTGTATAGAGAGCTCCCAGAA | 59 |
15 | TTAGACTGGATGTGCTCCAAC | AGAGGGACCCTGTGTTCTTT | 58 |
16 | CCCCTAGGGTCTTATGCAAG | GAGACTGGTCTTCCCCTGAA | 55 |
17 | CCCACTGGGCTGACTTTAT | AGGCTGGAGTTCTGGTCTCT | 56 |
18 | GGGCCTGAGCTAGATCATTT | AAGGGCTCAGGAATTCTCAT | 55 |
19 | GGCAGCTCTCATTCTTTCCT | TGAATATGGCCAAAGAGGAG | 60 |
20–21 | CATGCAGAGTGCTCTGAGTTAC | GGTCCATTTATGGGGTCTTC | 60 |
22 | TTCTAGGGAGGGGGTTTTAG | AAGCTTGTGAGCGACTCTTG | 59 |
23–24 | CCCAAGAGTCGCTCACAA | ACTCCTCGCTCCCATCTTC | 60 |
25 | CGAGCCTTTTCCTACATAAGC | CAGCACGAAGATGATGATTG | 58 |
26–27 | GGAATAGATCAAGAACCCCAGA | CTTCTTTGAAGCCTCCCTTG | 59 |
28 | TCTGTCCATTGGTTCCTCCT | TGTAGCTCCTGGTTTTGCTG | 60 |
29–30 | GGCCACTTCTTAGGGTTGTG | ACAGAAAAGGTGGAGGCAAC | 58 |
31 | GTAGGAACCGGAGGCAAG | CCTGGGGACAATCTCTGAC | 58 |
32–33 | GTGGGCTGTCAGAGATTGTC | CTACTTTGTCCCCAAACAGC | 59 |
34 | ATCTCCCAGAGGGTACCAGT | CCTGGGCGTATGTACTTGTC | 60 |
35 | TCCACAAGAATTTTATGATCCTC | TTGTCTTTTGTCTGGGAACC | 60 |
36 | TGTCCTTGAGTTGCTTGGTT | TCAGGATGATGGTGGATTGT | 59 |