Figure 2. mRNA transcripts of the β-tubulin (231 bp), nor-1 (168 bp) and pksA(395 bp) generated by RT–PCR from the corneal and environmental isolates of Aspergillus flavus. Ten µl of each amplified product were loaded on a 2% agarose gel. A: Lanes L1 and L7 were loaded with a 100 bp DNA marker. Lanes L2 to L5 were loaded with RT–PCR products of nor-1, pksA and β-tubulin genes from 7-day old cultures of corneal A. flavus isolates C1, C2, C4, and C7, respectively. Lanes L6 and L8 were loaded with controls for the PCR reaction. Lanes L9 to L12 were loaded with RT–PCR products of the nor-1, pksA and β-tubulin genes from 7-day old cultures of environmental A. flavus isolates E1, E4, E8, and E9, respectively. B: Graphical representation of the relative gene expression levels of the regulatory genes nor-1 and pksA of the corneal and environmental isolates of Aspergillus flavus.