Figure 1. mRNA transcripts of the β-tubulin (231 bp), aflR (514 bp) and aflJ (307 bp) generated by RT–PCR from the corneal and environmental isolates of Aspergillus flavus. Ten µl of each amplified product were loaded on a 2% agarose gel. A: Lanes L1 and L8 were loaded with controls for the PCR reaction. Lanes L2 and L7 were loaded with a 100bp DNA marker. Lanes L3 to L6 were loaded with RT–PCR products of aflR, aflJ and β-tubulin genes from 7-day old cultures of corneal A. flavus isolates C1, C2, C4 and C7, respectively. Lanes L9 to L12 were loaded with RT–PCR products of the aflR, aflJ and β-tubulin genes from 7-day old cultures of environmental A. flavus isolates E1, E4, E8 and E9, respectively. B: Graphical representation of the relative gene expression levels of the regulatory genes aflR and aflJ of the corneal and environmental isolates of Aspergillus flavus.