Figure 6. Effect of
CAV-1 promoter modification on the expression level in LUC system. Cells were transfected with 1 µg pGL3 promoterless LUC reporter
plasmids inserted with intact or modified 2,465 bases
CAV-1 promoter region (pGL3-CAV-1 promoterA or pGL3-CAV-1promoterG). LUC activity was averaged. The SEM is indicated by the error
bars. One – ratio of activity of construct with A to activity of construct with G in position −2,388 of
CAV-1 promoter; 2 – 8 putative cis-elements were deleted from
CAV-1 promoter and the activity of the construct with deleted sequence was expressed in percentage to the unmodified construct.
The following putative TF-binding sites were deleted: 2– NFκB (position −1537 −1527); 3 - HNF-3/Fkh homolog (position - 889
−882); 4 - POU-IV position (−807 −800); 5 – Sp-1 (position −236 −226); 6 – Ets (−182–174); 7 - SP-1 (position −124–115); 8–
NFkB (position −40 −28). Other details are presented in
Table 2.
A: The effect of CAV-1 promoter modification on LUC activity in HEK-293, SH-SY5Y and Y79 cells.
B: The effect of
CAV-1 promoter modification on LUC activity in NTM-5 and GTM-3 cells.