Figure 9 of Burke, Mol Vis 2011; 17:2864-2877.

Figure 9. Non-random distribution pattern in the time-dependent acquisition of nuclear propidium iodide (PI) fluorescence in ARPE-19 cells exposed to toxic doses of H2O2. Live cell imaging of dynamic changes in nuclear PI fluorescence in ARPE-19 cells following pulse delivery of H2O2. Images are bright-field-fluorescence overlays from two separate experiments illustrating nuclear PI staining (red) in cells at selected time intervals after exposure to row A: 750 μM or row B: 1000 μM H2O2. Images were captured at 5 min intervals after oxidant addition; the acquisition times of the selected images are indicated. The circled area identifies a region where earlier onset nuclear PI fluorescence has begun to fade 5 h later. Scale bar: 40 μm.