Figure 7 of Burke, Mol Vis 2011; 17:2864-2877.


Figure 7. Effect of photobleached melanosomes on the sensitivity of individual ARPE-19 cells to H2O2-induced toxicity compared to cells with a comparable content of control particles (latex beads) in co-cultures. Live cell imaging of dynamic changes in nuclear PI fluorescence over 18 h in ARPE-19 cells pre-loaded with latex beads or photobleached melanosomes (pb mel) and treated with H2O2 delivered as A: a pulse at 1500 μM or B: generated enzymatically by the addition of GOx at 40 mU/ml. Numbers of cells containing the different particle types selected for analysis were as follows. A: beads, n=59 (triangles); pb mel, n=63 (circles). B: beads, n=163 (triangles); pb mel, n=74 (circles). Data are from representative experiments and are the percent of the pre-selected cells in each particle group surviving (no nuclear PI) with time. The shift to the left of the curves illustrates the magnitude of the cytotoxic effect of pb mel relative to beads. The curves for the two particle types within each treatment protocol differ significantly (GraphPad Prism 5 survival analysis, p<0.02).