Figure 4 of Burke, Mol Vis 2011; 17:2864-2877.


Figure 4. Illustration of an imaging assay using co-cultures of ARPE-19 cells pre-loaded by phagocytosis with control latex beads or melanosomes. The same microscope field is shown by A: bright-field microscopy, and B, C: in merged bright-field and fluorescence images. The fluorescence image overlay in B shows latex bead autofluorescence (artificially colorized blue) and the double overlay in C also shows PI fluorescence (red), illustrating nuclear staining at 9 h and 20 min after addition of 750 μM H2O2. Circled cells in A show examples of cells pre-selected for analysis by their particle content. In B, the same cells are circled to illustrate discrimination of particle type in the selected cells; one contains beads (arrow) and the other melanosomes (double arrow). The arrow in C indicates a PI-positive nucleus in the bead-containing cell after H2O2 treatment. Scale bar: 20 μm.