Figure 5. Real-time RT–PCR of AQP4 and AQP5 from LCM samples of term pregnant rabbits. Table 2 compares these results to that of normal rabbit controls. mRNA levels for AQP4 was the lowest in interlobar duct, rather than acini as observed in control rabbits [5], and its level was significantly lower in all duct segments except interlobular duct (p<0.05). AQP5 mRNA was the highest in acini and was significantly lower (p<0.05) than that of normal control rabbits [5]. However, mRNA abundance was significantly increased in intralobular, interlobular, and interlobar ducts (p<0.05), while no change was found in intralobar duct. D4: intralobular duct. D3: interlobular duct. D2: intralobar duct. D1: interlobar duct. Data are presented as mean±SEM of 3 animals.