Figure 4 of Wang, Mol Vis 2011; 17:2835-2846.

Figure 4. Increasing TGFβ1 concentrations results in a loss of nuclear p38MAPK. HCFs were seeded on collagen in SSFM at 1×105 cells/ml in a 24 well dish. The next day cells were scratch-wounded and incubated in (A, E) SSFM, (B, F) 0.01 ng/ml TGFβ1, (C, G) 0.1 ng/ml TGFβ1, or (D, H) 1.0 ng/ml TGFβ. After 4 h cells were fixed and immunostained for p38MAPK (A-D) or SMAD 2/3 (E-H). Bar=50 μm. Arrows point to nuclei in which we detected either the nuclear localization or nuclear exclusion of p38MAPK and SMAD 2/3. The percent of cells in which p38MAPK was excluded from nuclei of the leading edge cells is shown in (I). The percent of cells with SMAD 2/3 concentrated in nuclei in the leading edge cells is shown in (J). Image J software was used for quantification. Each condition was compared to SSFM. ***p-value <0.001. Experiments were repeated at least three times with similar results.