Figure 3. Increasing TGFβ1 concentration promotes fibrotic markers. HCFs were seeded on collagen in SSFM at 1×10⁴ cells/ml on coverslips in a 24 well dish in (A) SSFM, (B) 0.01 ng/ml TGFβ1, (C) 0.1 ng/ml TGFβ1, or (D) 1.0 ng/ml TGFβ1. After 72 h (A-D) were immunostained for α-SMA. Bar=50 μm. For each condition, cells containing organized α-SMA stress fibers were counted (E) and using MetaMorph Analysis the relative cell area was quantified (F). For analysis greater than 100 cells per experiment were analyzed. Each condition was compared to SSFM, **p-value <0.01, ***p-value <0.001. Experiments were repeated at least three times with similar results.