concentrations reduces HCF migration. HCFs were seeded on
collagen in SSFM at 1×105
cells/ml in a 24 well dish.
The next day cells were scratch-wounded and incubated for 24 h
) SSFM, (B
) 0.01 ng/ml TGFβ1, (C
ng/ml TGFβ1, (D
) 1.0 ng/ml TGFβ1, or (E
) imaged at
time zero. Bar=200 μm. F
: Using T-Scratch
percent cell migration into the wound margin at 24 h compared to
time zero was calculated. Each condition was compared to SSFM,
*p-value <0.05, **p-value <0.01. Experiments were repeated
at least three times with similar results.