Figure 2. Increasing TGFβ1 concentrations reduces HCF migration. HCFs were seeded on collagen in SSFM at 1×10⁵ cells/ml in a 24 well dish. The next day cells were scratch-wounded and incubated for 24 h in (A) SSFM, (B) 0.01 ng/ml TGFβ1, (C) 0.1 ng/ml TGFβ1, (D) 1.0 ng/ml TGFβ1, or (E) imaged at time zero. Bar=200 μm. F: Using T-Scratch software, percent cell migration into the wound margin at 24 h compared to time zero was calculated. Each condition was compared to SSFM, *p-value <0.05, **p-value <0.01. Experiments were repeated at least three times with similar results.