Figure 5 of Su, Mol Vis 2011; 17:2798-2807.

Figure 5. Effect of crystallin peptides on the binding of α-crystallin to human lens membranes. Graphs showing the amount (ng) of Alexa350®-conjugated αA- (A) or αB-crystallin (B) bound per μg of human lens membrane in the presence of crystallin peptides. Human lens membranes (80 μg) were mixed with either 48 μg of peptide αB1–18 (solid bars), 48 μg of peptide γS167–178 (dotted bars), or with binding buffer only (horizontal line bars), for 30 min at 37 °C. Alexa350®-conjugated αA- or αB-crystallin (24 μg) was then added and the mixtures were incubated for 6 h at 37 °C. Results are the mean±SEM (n=3). *p<0.05, **p<0.01, ***p<0.001 compared with assays performed with binding buffer only (horizontal line bars).