Figure 5. Effect of crystallin peptides on the binding of α-crystallin to human lens membranes. Graphs showing the amount (ng) of Alexa350^®-conjugated αA- (A) or αB-crystallin (B) bound per μg of human lens membrane in the presence of crystallin peptides. Human lens membranes (80 μg) were mixed with either 48 μg of peptide αB_1–18 (solid bars), 48 μg of peptide γS_167–178 (dotted bars), or with binding buffer only (horizontal line bars), for 30 min at 37 °C. Alexa350^®-conjugated αA- or αB-crystallin (24 μg) was then added and the mixtures were incubated for 6 h at 37 °C. Results are the mean±SEM (n=3). *p<0.05, **p<0.01, ***p<0.001 compared with assays performed with binding buffer only (horizontal line bars).