Figure 4. Binding of α-crystallin to human lens membrane preparations. Binding curves depicting the amount of bound Alexa350^®-conjugated αA- (A) and αB-crystallin (B) per μg of urea-treated cortical (●) and nuclear (▲) human lens membrane, or alkali-treated cortical (○) and nuclear (△) human lens membrane as a function of the free α-crystallin concentration. Human lens membranes (80 μg) were incubated with a mass range of Alexa350^®-conjugated αA- and αB-crystallins, for 6 h at 37 °C. Results are the mean±SEM (n=3).