Figure 3. Binding of α-crystallin to bovine lens membrane preparations. Binding curves depicting the amount of bound Alexa350^®-conjugated αA- (A) and αB-crystallin (B) per μg of urea-treated (●) or alkali-treated (▲) bovine lens membrane as a function of the free α-crystallin concentration. Bovine lens membranes (200 μg) were incubated with a mass range of Alexa350^®-conjugated αA- and αB-crystallins, for 6 h at 37 °C. Results are the mean±SEM (n=3).