Figure 1. SDS–PAGE and western immunoblot analysis of bovine lens membranes. The water-insoluble cell membrane pellets from bovine lens homogenates washed with 7 M urea (BU) and with 0.1 M NaOH (BA). Samples were analyzed by SDS–PAGE (100 μg of membrane per lane) and western immunoblotting (20 μg of membrane per lane). Blots were probed using anti-αA-crystallin, anti-αB-crystallin, anti-filensin and anti-vimentin antibodies, as indicated. Molecular weight markers are on the left of the figure.