Figure 6. Skp2 siRNA inhibited the cell proliferation of PFC in vitro (Brdu). For Brdu incorporation, cells growing on coverslips were incubated
with Brdu. Incorporated Brdu was detected with antibodies as described in the Methods. Statistical analysis after cell counting
showed that Brdu positive cells decreased after hPFC transfection with Skp2 siRNA compared with control cells. **p<0.01 versus vehicle and blank control.