Figure 2. Down-regulation of Skp2 protein by siRNA in PFC in vivo by immunoflurescence. Immunoflurescence staining indicated high constitutive
levels of Skp2 protein in the nucleolus of PFC and NFC transfected with pSuppressor vehicle (A, D) or without transfection (B, E). Transfection with Skp2 siRNA dramatically decreased the expression of Skp2 protein in PFC cells (C, F). The scale bar is equal to 40 μm.