Figure 1. Eukaryotic translation initiation factor 5A (eIF5A) expression in human corneal epithelial cells (HCEpiC) was assessed in this experiment. A: Relative eIF5A gene expression levels at 18 h after administration of epidermal growth factor (EGF; 10 ng/ml) and medium alone (control) was detected by real-time polymerase chain reaction (PCR). The amplification plot of eIF5A and β-actin (ACTB) are shown. The graph shows the relative eIF5A protein levels normalized to β-actin. (**) were highly significant when compared with control group, in both cases p-value <0.01. B: The expression of eIF5A protein was measured by using western blot analysis. The graph shows the relative eIF5A protein levels normalized to β-actin. Results are presented as the mean±standard deviation of three independent experiments (n=3), each conducted in triplicate. (**) were highly significant when compared with control group, in both cases p-value <0.01.