Figure 7 of Hollborn, Mol Vis 2011; 17:2738-2750.


Figure 7. Light-evoked calcium responses in cultured Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1) cells. A: Responses of cells with dark adaptation are shown. B, C: Responses of cells without previous dark adaptation are shown. The cells were repetitively illuminated with light of 480 nm (A, B) and 600 nm (C), respectively. The traces represent the light emission alterations of the calcium-sensitive dye, Fluo-4, recorded between 505 nm and 550 nm; an increase in the fluorescence ratio reflects an elevation in cytosolic free calcium. The relative fluorescence change over time was calculated relative to the fluorescence measured at the beginning of the light stimulation (ΔF/F0) and is given in percentages. Each trace represents the calcium response of one cell. Arrows indicate fast transient responses.