Figure 7. Light-evoked calcium
responses in cultured Moorfields/Institute of
Ophthalmology-Müller 1 (MIO-M1) cells. A: Responses of
cells with dark adaptation are shown. B, C:
Responses of cells without previous dark adaptation are shown.
The cells were repetitively illuminated with light of 480 nm (A,
B) and 600 nm (C), respectively. The traces
represent the light emission alterations of the
calcium-sensitive dye, Fluo-4, recorded between 505 nm and 550
nm; an increase in the fluorescence ratio reflects an elevation
in cytosolic free calcium. The relative fluorescence change over
time was calculated relative to the fluorescence measured at the
beginning of the light stimulation (ΔF/F0) and is
given in percentages. Each trace represents the calcium response
of one cell. Arrows indicate fast transient responses.