Figure 6. Detection of melanopsin and blue opsin in Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1) cells and human retinas by western blot analysis. A: Melanopsin (Me; 65 kDa) was found in the cytosolic (Cyt) and membrane (Mem) fractions of MIO-M1 cells. As the control, the presence of melanopsin was detected in neural retinal tissues from two postmortem donors (1, 2). The blots were stained with (a) and without (b) the first antibody. B: Presence of blue opsin (BO; 40 and 42 kDa) in the cytosolic (Cyt) and membrane (Mem) fractions of MIO-M1 cells and in two neural retinas (R1, R2), respectively (a). The isotype control (b) was made with purified rabbit IgG.