Figure 2. Expression of neuronal and
stem cell marker genes in Moorfields/Institute of
Ophthalmology-Müller 1 (MIO-M1) cells. The expression of the
following neuronal genes was determined with reverse
transcription PCR: Thy-1 (THY1), neurofilament heavy
polypeptide (NEFH), neurogenic differentiation 1 (NEUROD1),
microtubule-associated protein 2 (MAP2), and neuronal
nuclei (NEUN). The expression of the following neural
progenitor genes was determined: nestin (NES),
paired-type homeobox transcription factor (PAX6), and
neurogenic locus notch homolog protein 1 (NOTCH1). The
data from two independent experiments (1, 2) are shown. The
negative control (-) was done by adding double-distilled water
instead of cDNA.