Figure 13. Microscopic analysis of uptake of nanoparticles by Y79 cells. Fluorescent microscopic analysis showing the uptake of native coumarin (A-D), coumarin encapsulated nanoparticles (COU-NP; E-H), and coumarin encapsulated nanoparticles conjugated with epithelial cell adhesion molecule antibody (COU-NP-EpCAM; K-N) at 4 h (A, E, K), day 1 (B, F, L), day 2 (C, G, M), and day 5 (D, H, N). The Y79 cells showed increased COU-NP-EpCAM uptake compared to unconjugated COU-NP at all time points. Increased coumarin-NP retention was observed in the Y79 cells even at day 5 when compared to free coumarin.