Figure 11. Cell cycle analysis of Y79 cells treated with paclitaxel loaded nano-conjugates. Increased G2-M arrest was observed by flow cytometry analysis in Y79 cells treated with paclitaxel-loaded nanoparticles conjugated with epithelial cell adhesion molecule antibody (PTX-NP-EpCAM) at 48 h compared to paclitaxel-loaded nanoparticles (PTX-NP) or native paclitaxel (PTX). A: Untreated Y79 cells showing 3.8% of G2-M phase after doublet discrimination. B: Y79 cells treated with native paclitaxel showing increased G2-M cells (12.2%). C: Y79 cells treated with PTX-NP showing 17.8% of G2-M phase. D: Y79 cells treated with PTX-NP-EpCAM showing 21.7% G2-M phase.