Table 1 of
Mackay, Mol Vis 2011; 17:2706-2716. Table 1 of
Mackay, Mol Vis 2011; 17:2706-2716.
Table 1. Primers and PCR conditions used in screening RDH12 in this cohort.
Exon |
Primer |
PCR annealing (°C) |
Size of fragment (bp) |
|---|---|---|---|
| Exon 1F | TTTCCCCACATTCTCTTTGCC | 54 | 517 |
| Exon 1R | TCCACCATGGTATCCACAACACC | ||
| Exon 2F | TAACGTATCTTAGTGTGAGCTCG | 54 | 306 |
| Exon 2R | TCCTTGAATTTCTAGTCAGAGC | ||
| Exon 3F | TCACTCTACCGTTGAAGGATGG | 54 | 405 |
| Exon 3R | TGTGGCAGAACCTGTCTAGTGG | ||
| Exon 4F | ATAGTTATTGAGTGCTGAGGC | 54 | 459 |
| Exon 4R | TAGACTGATCAGGAGAGGTAC | ||
| Exon 5F | TCAGACCAAACTGACCATTAGAG | 54 | 460 |
| Exon 5R | TGACACGTGCATGTTTGACAGCC | ||
| Exon 6F | TGGTACCTGCTGAATCCTGGG | 54 | 434 |
| Exon 6R | ACCTGGATTGCATCATCAGGC | ||
| Exon 7F | TTAGTTTCTTTGAGTCTGGC | 54 | 884 |
| Exon 7R | TGATTTGTTCCATTTCTCTCC |