Table 2 of
Ju, Mol Vis 2011; 17:2698-2705. Table 2 of
Ju, Mol Vis 2011; 17:2698-2705.
Table 2. Primers of plasmid which was constructed for yeast two-hybrid system.
Plasmid |
Primers |
Vectors |
Enzyme cut sites |
Fragment size |
|---|---|---|---|---|
| Pax-6- pGBK-T7 | 5′-GGAATTCCATATGATGCAGAACAGTCACAGCGG-3′ | pGBK-T7 | Nde1, EcoR1 | 1288 |
| 5′-CCGGAATTCCTGTAGTCTTGGCCAGTACTG-3′ | ||||
| Pax-6-pGAD-T7 | 5′-CCGGAATTCATGCAGAACAGTCACAGCGG-3′ | pGAD-T7 | EcoR1, Xho1 | 1285 |
| 5′-CCGCTCGAGCCTGTAGTCTTGGCCAGTACTG-3′ | ||||
| Pax-6-variant-pGAD-T7 | 5′-CCGGAATTCATGCAGAACAGTCACAGCGG-3′ | pGAD-T7 | EcoR1, Xho1 | 1198 |
| 5′-CCGCTCGAGCTAAAATACTGCTGAACATCC-3′ | ||||
| Pax-6-variant- pGBK-T7 | 5′-GGAATTCCATATGATGCAGAACAGTCACAGCGG-3′ | pGBK-T7 | Nde1, EcoR1 | 1201 |
| 5′-CCGGAATTCTAAAATACTGCTGAACATCC-3′ |