Table 1 of
Ju, Mol Vis 2011; 17:2698-2705. Table 1 of
Ju, Mol Vis 2011; 17:2698-2705.
Table 1. Primers of plasmid which was constructed for protein purification.
Plasmid |
Primers |
Vector |
Enzyme cut sites |
Fragment size |
|---|---|---|---|---|
| pET-28-Pax-6 | 5′-CCGGAATTCATGCAGAACAGTCACAGCGGA-3 | pET28 | EcoR I, Hind III | 1270 |
| 5′-CCCAAGCTTCTGTAGTCTTGGCCAGTACTG-3′ | ||||
| pET-28-Pax-6 variant | 5′-CCGGAATTCATGCAGAACAGTCACAGCGGA-3 | pET28 | EcoR I, Hind III | 1182 |
| 5′-AAAAAGCTTTAAAATACTGCTGAACATCC-3′ | ||||
| pET-28-Gfp-Pax-6 | 5′-AAACCATGGGTAAAGGAGAAGAAC-3 | pET28 | NcoI, Hind III | 948 |
| 5′GTCTGGCTGGGTACAGGGGGTTTGTATAGTTCATCCATG-3′ | ||||
| 5′CATGGATGAACTATACAAACCCCCTGTACCCAGCCAGAC-3′ | ||||
| 5′TTTAAGCTTCTGTAGTCTTGGCCAGTACTG-3′ | ||||
| pET-28-Gfp-Pax-6-variant | 5′-AAACCATGGGTAAAGGAGAAGAAC-3′ | pET28 | NcoI, Hind III | 864 |
| 5′-CTCCAGGGGAAATGAGACTTTGTATAGTTCATCCATG-3′ | ||||
| 5′-CATGGATGAACTATACAAAGTCTCATTTCCCCTGGAG-3′ | ||||
| 5′-AAAAAGCTTTAAAATACTGCTGAACATCC-3 |