Figure 1. Identification of keratocytes. Inverted phase-contrast images (A) and immune florescent vimentin staining (B) of human keratocytes. Keratocytes were observed growing parallel to the etched lines. Immunofluorescence with anti-vimentin antibody, a general marker of keratocyte, showed the morphology. The nuclei were stained with Hoechst 33342 (blue). Magnification, 200×.