Figure 4. Label-free imaging of TM
cells using CARS and collagen extracellular matrix using TPAF.
The image is taken using a 60× 1.2 NA water objective with 3×
digital zoom. (RED: CARS. Green: TPAF). A: Both CARS and
TPAF channels are displayed in the image, clearly showing the TM
cells in the CARS channel with arrows indicating the TM cells. B:
Only the TPAF channel is displayed and TM cells are not
observed, indicating that signal of the TM cells are unique to
the CARS signal.